In melanin synthesis, tyrosinase catalysis has two distinct reactions: the hydroxylation of L-tyrosine to L-dopa and the oxidation of L-dopa to dopaquinone. Dopaquinone after inherent conversion becomes dopachrome, and dopachrome tautomerase (tyrosinase-related protein-2, DCT/TRP-2) catalyzes the conversion of dopachrome to 5,6-dihydroxyindole-2-carboxylic acid (DHICA). By DHICA oxidase (TRP-1), DHICA is converted to indole-quinone-carboxylic acid. The tyrosinase-related proteins, TRP-1, and TRP-2 catalyze distal melanin synthesis steps, which control the type of melanin produced [ 21 , 22 ]. After we added the L-tyrosine as the substrate, the serial reaction was the hydroxylation of L-tyrosine to L-dopa and the oxidation of L-dopa to dopaquinone. We tested the dopaquinone at 490 nm, and the final concentration of DMSO was at %.